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  • Title: Affinity chromatography of yeast alcohol dehydrogenase using immobilized monochlorotriazine colourless compounds.
    Author: Li Y, Kunyu G, Lubai C, Hanfa Z, Yukui Z.
    Journal: Biomed Chromatogr; 1997; 11(3):180-4. PubMed ID: 9192114.
    Abstract:
    Four new symmetrical colourless biomimetics bearing two monochlorotriazine rings have been rationally designed and synthesized. These immobilized colourless ligands and one analogue of Cibacron Blue F3GA on Sepharose CL-4B were used to purify alcohol dehydrogenase from baker's yeast extract. Twenty-two-fold purification with 78% enzyme recovery was achieved in a single step with specific elution of NAD+ (5 mM) from the colourless absorbent comprised of two ortho-aminobenzene sulphonates as terminal rings, which is comparable to the result obtained from the analogue of Cibacron Blue F3GA. Differential spectra between ligand-enzyme complexes and free ligands were studied.
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