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  • Title: [Scanning electron microscopic studies on mucosal coating by barium mixtures].
    Author: Usuda K.
    Journal: Nihon Igaku Hoshasen Gakkai Zasshi; 1997 May; 57(6):326-9. PubMed ID: 9194362.
    Abstract:
    The purpose of this study was to observe and evaluate mucosal coating by barium mixtures using electron microscopy. Specimens from the ileum of two pigs, about 50cm in length from the terminal ileum, were divided into 3 groups with different in concentrations and additives of barium mixture. The 50cm length of ileum was cut into 10cm long pieces. Four specimens from each group were evaluated by double contrast study for coating by the barium layer. Then the specimen was frozen with liquid nitrogen solution, and fixed. Afterward, the specimen was cut into about 1cm sections, fixed again, dehydrated, dried, and observed by scanning electron microscopy (SM). In the double contrast study, the differences in barium coating were correlated with the concentration of barium sulphate. The scanning electron microscopic findings were correlated with those of the double contrast study. The various additives in the barium mixture did not cause any distinctive differences. Although barium particles have been studied by electron microscopy, barium mixture coating state on the mucosa of biological materials has not been reported. In conclusion, a correlation of the coated barium layer on double contrast study and the images of scanning electron microscopy was observed using pig ileum specimens.
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