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  • Title: [The complex detection of AB(O)-system antigens and of keratin polymorphism in the forensic medical study of hair].
    Author: Kukhar'kov IuV, Puchkov GF, Borovko SR.
    Journal: Sud Med Ekspert; 1997; 40(1):31-3. PubMed ID: 9198980.
    Abstract:
    Hair proteins were extracted by buffer consisting of 8 M urea, 0.025 M dithiothreitol, 2% sodium dodecylsulfate, and 0.01 M tris-HCl pH 10.4. The proteins were analyzed by gradient polyacrylamide gel electrophoresis. A single 5 cm hair, previously subjected to AB0 blood group analysis by the absorption-elution test, was sufficient for assessing the keratin phenotype. It is possible to determine the AB(0) antigens and detect the keratin phenotype in the same hair fragments.
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