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  • Title: Effect of hormones on expression of prolactin receptor messenger ribonucleic acids in pancreatic islets of adult female mice in vitro.
    Author: Matsuda M, Mori T.
    Journal: Zoolog Sci; 1997 Feb; 14(1):159-65. PubMed ID: 9200989.
    Abstract:
    We studied the effects of hormones on expression of prolactin receptor (PRL-R) mRNA in pancreatic islets of adult female mice in vitro. We quantified mRNA expression in small amounts of the islet tissue by competitive PCR and one-sided competitive PCR. Fifty pancreatic islets from adult female mice were cultured in a wall for 4 days with or without ovine prolactin (PRL), bovine growth hormone or estradiol-17 beta. PRL (1 microgram/ml) significantly increased the insulin secretion and the amount of PRL-R mRNA relative to that of beta-actin mRNA. Growth hormone (1 microgram/ml) also increased the relative amount of PRL-R mRNA, although it did not significantly increase the insulin secretion. Neither insulin secretion nor the relative amount of PRL-R mRNA was affected by estradiol (100 ng/ml). The ratio of the short form to the long form of PRL-R mRNA was not altered by these hormones. The present observation that PRL increased PRL-R mRNA expression in pancreatic islets thus suggests the possibility that PRL up-regulates the tissue sensitivity to PRL itself during lactation.
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