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Title: Isolation and characterization of isoenzymes of human salivary and pancreatic alpha-amylase. Author: Rosenmund H, Kaczmarek MJ. Journal: Clin Chim Acta; 1976 Sep 06; 71(2):185-9. PubMed ID: 9210. Abstract: Human salivary and pancreatic alpha-amylase (1,4-glucan 4-glucanohydrolase, EC 3.2.1.1) were separated by electrofocusing. In the first case we obtained six isoenzymes with isoelectric points of pH 5.70, 5.72, 6.23, 6.32, 6.73 and 6.88. Human pancreatic alpha-amylase has been separated into eight isoenzymes with isoelectric points of pH 5.72, 5.77, 5.88, 6.05, 6.23, 6.69, 6.72 and 6.95. Some of the isoenzymes were shown to be sialoproteins; others representing about 80% of the total activity did not contain neuraminic acid. The molecular weight of the non-sialoproteinic isoenzymes was found to be about 47 000 in all cases.[Abstract] [Full Text] [Related] [New Search]