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  • Title: Separation of pig bone alkaline phosphatase activities.
    Author: Leunis JC, Vancraeynest T, Brauman J.
    Journal: Ann Biol Clin (Paris); 1977; 35(2):89-95. PubMed ID: 921014.
    Abstract:
    A simple method for the separation of alkaline phosphatase and pyrophosphatase activities of pig bone ribs is described. Using anionic exchange chromatography (DEAE-cellulose) and affinity chromatography on Concanavalin A sepharose (Con A) eluted by a step pH gradient and Na4P2O7, several activities were obtained. A pyrophosphatase containing very little alkaline phosphatase activity was isolated from Con A sepharose by elution with pyrophosphatase. Our data are consistent, with the hypothesis that cortical alcaline phosphatase and pyrophosphatase activities are not due to a single enzyme protein. The method was used on whole bone, on bone marrow and on cortical bone.
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