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  • Title: Use of zonal centrifugation method for the preparation of mu-opioid receptor enriched membranes from bovine corpus striatum.
    Author: Sastry KV, Harikumar KG, Sailaja P, Janardana Sarma MK.
    Journal: J Neurosci Methods; 1997 Jun 06; 74(1):45-52. PubMed ID: 9210574.
    Abstract:
    Earlier attempts to purify the opioid receptors met with limited success because of the use of brain crude membranes. Subcellular fractionation procedures adopted now to get enriched membranes resulted in 2-3 fold enrichment. However, a procedure has been developed in our laboratory in which a crude membrane fraction obtained at 17,500 x g was lysed with 1 mM sodium bicarbonate and later subjected to zonal fractionation, employing a density gradient of 0.6-1.2 M sucrose. This could yield a membrane fraction highly enriched with mu-opioid receptors which is 9.3 fold higher than the crude membranes.
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