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  • Title: [Fractionation of chromatin in acrylamide gel with immobilized non-histone proteins and DNA].
    Author: Kharuenko EP, Shestak Ki, Ashmarin IP.
    Journal: Biokhimiia; 1977 Oct; 42(10):1881-7. PubMed ID: 922074.
    Abstract:
    Liver and kidney chromatin and DNA were fractionated in acrylamide columns with immobilized readily extracted non-histone chromosomal proteins (RE-NHCP). Chromatin was fractionated in two fractions on the column with RE-NHCP, one of the fractions is sorbing and has a poor protein contents, as compared to the initial chromatin preparation. Fractionation of chromatin in two fractions (sorbing and non-sorbing) was also observed under its chromatography in the column with immobilized DNA. Both chromatin fractions are stripped in the protein content after the elution, which is probably due to the transfer of some proteins from chromatin to immobilized DNA. Chromatography of DNA on gel with homologous RE-NHCP also results in DNA fractionation into sorbing and non-sorbing fractions. The data obtained suggest the irregularity of the distribution and the restriction of "binding sites" for exogenous homologous and heterologous RE-NHCP and of the number of chromatin proteins, which are capable of additional binding with exogenous homologous DNA.
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