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  • Title: Application of immobilized chymotrypsin in a multistage fluidized-bed reactor.
    Author: Halwachs W, Wandrey C, Schügerl K.
    Journal: Biotechnol Bioeng; 1977 Nov; 19(11):1667-77. PubMed ID: 922129.
    Abstract:
    The stereospecific hydrolysis of D,L-phenylalanine methylester with immobilized alpha-chymotrypsin was carried out as a model reaction for the racemate resolution of aromatic amino acids in a five staged fluidized-bed reactor (FBR). Owing to ester hydrolysis, a pH shift occurred along the reactor. Because of the pH-dependent enzyme activity a particular longitudinal pH profile had to be enforced by a proper entrance pH in order to gain an optimum conversion. In the FBR with optimum pH profile, higher conversions were achieved than in a continuous stirred tank reactor (CSTR) at the pH optimum and at the same contact time. By the application of a proton balance and the results of kinetic measurements a model was developed for the prediction of the optimum longitudinal pH profile with regard to the maximum conversion.
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