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  • Title: Dual multimodular class A penicillin-binding proteins in Mycobacterium leprae.
    Author: Lepage S, Dubois P, Ghosh TK, Joris B, Mahapatra S, Kundu M, Basu J, Chakrabarti P, Cole ST, Nguyen-Distèche M, Ghuysen JM.
    Journal: J Bacteriol; 1997 Jul; 179(14):4627-30. PubMed ID: 9226276.
    Abstract:
    The ponA gene of cosmid L222 of the Mycobacterium leprae genome library encodes a multimodular class A penicillin-binding protein (PBP), PBP1. The PBP, labelled with a polyhistidine sequence, has been produced in Escherichia coli, extracted from the membranes with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane-sulfonate (CHAPS) and purified by Ni2(+)-nitrilotriacetic acid-agarose chromatography. In contrast to the pon1-encoded class A PBP1, PBP1 undergoes denaturation at temperatures higher than 25 degrees C, it catalyzes acyl transfer reactions on properly structured thiolesters, and it binds penicillin with high affinity.
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