These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A rapid assay for urinary porphyrins by dual-wavelength spectrophotometry.
    Author: Christensen NG, Johannessen B, Romslo I.
    Journal: Clin Chim Acta; 1977 Dec 15; 81(3):229-35. PubMed ID: 923095.
    Abstract:
    A procedure has been developed for the separation and quantitative assay of urinary porphyrins. Urine was directly diluted to a final concentration of 1 MHCl and the amount of porphyrins was determined from the peak-to-trough height deflection of the first derivatives of the absorption spectrum in the region of the Soret band. The ratio uro-/coproporphyrin was determined from the wavelength at which the spectrum intercepted the baseline. The specificity (as shown by a correlation coefficient of 0.99 compared to the method of Doss and Schmidt (1971) Z. Klin. Chem. Klin. Biochem. 9, 415-418), precision (coefficient of variation 5.2 percent) and sensitivity (lower detection limit approx. 0.01 mumol/l) of the present method were highly sufficient to estimate total and different porphyrins in the routine laboratory.
    [Abstract] [Full Text] [Related] [New Search]