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  • Title: Improved in vitro cultivation of Babesia caballi.
    Author: Avarzed A, Igarashi I, Kanemaru T, Hirumi K, Omata Y, Saito A, Oyamada T, Nagasawa H, Toyoda Y, Suzuki N.
    Journal: J Vet Med Sci; 1997 Jun; 59(6):479-81. PubMed ID: 9234227.
    Abstract:
    Babesia caballi infected erythrocytes were collected from the blood of an experimentally infected horse and could be continuously cultivated in vitro with parasitemia ranging from 2-4% in RPMI 1640 medium supplemented with 2 mM L-glutamine, 20 mM HEPES and 40% adult horse serum in a low oxygen atmosphere (2% O2, 5% CO2 and 93% N2). All attempts to increase parasitemia failed using other culture media, serum concentrations and culture vessels. However, parasite growth was enhanced by transfer of cultures from a low oxygen to 5% CO2 in air, with parasitemia ranging from 8-10%.
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