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  • Title: Identification of a biological inactive complex form of pokeweed antiviral protein.
    Author: Desvoyes B, Poyet JL, Schlick JL, Adami P, Jouvenot M, Dulieu P.
    Journal: FEBS Lett; 1997 Jun 30; 410(2-3):303-8. PubMed ID: 9237651.
    Abstract:
    Pokeweed antiviral protein (PAP) inactivates both eukaryotic and prokaryotic ribosomes via a specific depurination of rRNA. The sensitivity of pokeweed ribosomes to PAP implies the existence of a mechanism to protect the plant. Using monoclonal antibodies specific to PAP, a protein complex (PAPi) which contained PAP was identified in leaf extract. In this complex, the enzymatic activity of the toxin was strongly inhibited. This protein complex had a pI lower than that of PAP and was separated from free PAP by a preparative native gel electrophoresis. PAPi had an apparent molecular mass of 57 kDa and was dissociated by heating for 5 min at 80 degrees C or by treatment by alkaline or acidic pH or by 7 M urea. The other components involved in the complex remain unknown.
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