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  • Title: Rapid screening of multiple forensic stains by SSCP analysis of HLA-DQ alpha amplification products.
    Author: Kreike J, Lehner A.
    Journal: J Forensic Sci; 1997 Jul; 42(4):715-7. PubMed ID: 9243838.
    Abstract:
    Single strand conformation polymorphism (SSCP) analysis was performed with HLA-DQ alpha amplification products. The various HLA-DQ alpha genotypes reveal different SSCP patterns. These are reproducible in DNA preparations from unrelated persons with identical HLA-DQ alpha genotype. However, in one of the more frequent HLA-DQ alpha genotypes (1.1/4) two variant SSCP patterns were observed. SSCP analysis allows rapid low-cost screening of many specimens and is an alternative discrimination test of stains beside reverse dot blot hybridization.
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