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  • Title: In vitro exocytosis in sea urchin eggs requires a synaptobrevin-related protein.
    Author: Avery J, Hodel A, Whitaker M.
    Journal: J Cell Sci; 1997 Jul; 110 ( Pt 14)():1555-61. PubMed ID: 9247189.
    Abstract:
    Sea urchin eggs provide an efficient in vitro model of exocytosis. We have identified proteins in sea urchin eggs that cross-react with antibodies to mammalian synaptobrevin, synaptotagmin, SNAP-25, syntaxin and rab3a. We show that these proteins are localized to the sea urchin egg cortex, using western blotting and immunocytochemistry. Tetanus toxin light chain cleaves the synaptobrevin-related protein in vitro and inhibits calcium-induced exocytosis. These data demonstrate a conservation between phyla of protein sequence and molecular mechanisms thought to facilitate exocytosis and show that the sea urchin egg provides a unique in vitro exocytotic model with which to study the conserved protein machinery of membrane fusion during secretion.
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