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  • Title: Quantitative evaluation of fluorescence in situ hybridization (FISH) signals in uncultured coelomic cells.
    Author: Crüger DG, Bruun-Petersen G, Kølvraa S.
    Journal: Prenat Diagn; 1997 Jul; 17(7):635-42. PubMed ID: 9249864.
    Abstract:
    Uncultured coelomic cells were hybridized with alpha satellite DNA probes representing chromosomes X, Y, 18, and 13/21 in order to evaluate the distribution of hybridization signals obtained by fluorescence in situ-hybridization (FISH) analysis of this cell type. Cells from 26 samples were hybridized with the X probe and the Y probe was hybridized with cells from 25 of the samples. Cells from 16 and 11 samples were hybridized with an 18 alpha satellite DNA probe and 13/21 alpha satellite probe, respectively. The evaluation demonstrated that FISH with X, Y, 18, and 13/21 alpha satellite DNA probes on uncultured coelomic cells is a reliable technique since the distribution of hybridization signals is comparable to that seen in uncultured amniotic fluid cells.
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