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  • Title: A triple stain for the detection of Helicobacter pylori in gastric brushing cytology.
    Author: Ghoussoub RA, Lachman MF.
    Journal: Acta Cytol; 1997; 41(4):1178-82. PubMed ID: 9250318.
    Abstract:
    OBJECTIVE: Helicobacter pylori is a surface bacterium associated with gastritis. The triple stain (TS), combining silver, hematoxylin and eosin, and alcian blue at pH 2.5, is valuable in the detection of H pylori in tissue. We evaluated the usefulness of TS as compared with Papanicolaou stain in detecting H pylori in gastric brushings and biopsy specimens. STUDY DESIGN: Gastric brushings and biopsy specimens were obtained from 21 patients. The Papanicolaou-stained slides were restained with TS, and the brushings and biopsy specimens were independently evaluated by the authors. RESULTS: H pylori was found in 9 of 21 randomly selected cases using TS. Of these, two cases were positive on brushings alone and three on biopsy alone. Only three of the nine cases were positive by Papanicolaou stain. None of the 21 samples were positive with Papanicolaou stain yet negative with TS. Detection of H pylori was restricted to patients with gastric ulcerations but was not limited by paucity of glands. CONCLUSION: TS is useful for detecting H pylori in gastric brushings and provides excellent cytologic detail. Furthermore, TS is superior to Papanicolaou stain for H pylori detection and is a valuable adjunct to biopsy.
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