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  • Title: Cloning and expression of an endo-1,3-1,4-beta-glucanase gene from Bacillus macerans in Lactobacillus reuteri.
    Author: Heng NC, Jenkinson HF, Tannock GW.
    Journal: Appl Environ Microbiol; 1997 Aug; 63(8):3336-40. PubMed ID: 9251227.
    Abstract:
    Strains of the gastrointestinal species Lactobacillus reuteri were electrotransformed with plasmid constructs containing the endo-1,3-1,4-beta-glucanase gene (bglM) of Bacillus macerans. The enzyme was expressed and secreted by the lactobacilli. A plasmid construct containing the bglM gene lacking its promoter was derived and was demonstrated to be useful as a promoter probe vector.
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