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  • Title: Agarose gel electrophoresis of cerebrospinal fluid proteins and analysis of the pherogram profiles by analog computer.
    Author: Siegert M, Siemes H.
    Journal: J Clin Chem Clin Biochem; 1977 Nov; 15(11):635-44. PubMed ID: 925629.
    Abstract:
    A micro-method of agarose gel electrophoresis requiring 1--3 ml of cerebrospinal fluid for quantitative analysis of cerebrospinal fluid proteins is described. After concentration of CSF to about 50 microliter by ultrafiltration and refractometric determination of protein, approximately 20--40 microgram of total protein are used for electrophoresis. Photometric scanning of the electrophoretic pattern at two different wavelengths permits quantitative evaluation. The pherograms are analysed by means of a modified DU-PONT analog computer. Factors which influence quantitative electrophoresis are examined. In cerebrospinal fluid of normal children 15 protein fractions are demonstrated: 2 prealbumins, albumin, 5 alpha-, 3 beta- and 4 gamma-globulins.
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