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  • Title: Differentiation of dual seropositivity to HIV 1 and HIV 2 in Ghanaian sera using line immunoassay (INNOLIA).
    Author: Mingle JA.
    Journal: West Afr J Med; 1997; 16(2):71-4. PubMed ID: 9257539.
    Abstract:
    Two hundred serum samples from AIDS-like patients, blood donors and miscellaneous patients were screened by ELISA for HIV 1-2. Repeatedly positive samples were confirmed using the Western Blot (WB). Twenty samples that were dual positive were further analysed by Innolia, which contains synthetic or recombinant antigens in HIV-1 and HIV-2. Only 5 of the samples were dual positive. The need for further analysis of dual WB results is re-emphasized. A serologic study conducted in Ghana highlighted the importance of differentiating between HIV cross-reactivity and specific reactions directed against the virus itself. 200 serum samples collected from blood donors, patients suspected to have AIDS, and other patients were screened by enzyme-linked immunosorbent assay (ELISA) and, if reactive, confirmed by Western blot. The 20 samples that were dually positive for HIV-1 and HIV-2 according to Western blot were further analyzed by INNOLIA, which contains synthetic or recombinant antigens to HIV-1 and HIV-2. Only 5 of the 20 samples were dually positive. 10 samples were positive for HIV-1 alone, 4 were positive for HIV-2 alone, and 1 was indeterminate. The INNOLIA test offers a more specific test for differentiation than the Western blot, and it is simpler to use since results for both HIV-1 and HIV-2 are recorded on a single test strip.
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