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  • Title: Alpha 5 subunit forms functional alpha 3 beta 4 alpha 5 nAChRs in transfected human cells.
    Author: Fucile S, Barabino B, Palma E, Grassi F, Limatola C, Mileo AM, Alemà S, Ballivet M, Eusebi F.
    Journal: Neuroreport; 1997 Jul 28; 8(11):2433-6. PubMed ID: 9261804.
    Abstract:
    nAChRs heterologously expressed in human cells after transient transfection with alpha 3 beta 4 alpha 5 or alpha 3 beta 4 subunit cDNAs exhibited similar sensitivities to antagonists and comparable functional channel profiles. However, the sum of two Hill equations was required for best fitting the ACh dose-current response curves after co-expression of alpha 5, alpha 3 and beta 4 subunits. One component was comparable to that obtained in alpha 3 beta 4-transfected cells, while the additional component, putatively attributed to an alpha 3 beta 4 alpha 5 nAChR population, showed a Hill coefficient > 2 and a nine-fold greater half-maximal ACh concentration (EC50). These results suggest that the alpha 5 subunit participates in the assembly of alpha 3 beta 4 alpha 5 nAChRs complexes in human cells, adding a new member to the family of neuronal nicotinic receptors.
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