These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Expression and regulation of mRNA for inhibin/activin alpha- and betaA-subunits in the granulosa layer of the two largest preovulatory follicles during the hen ovulatory cycle.
    Author: Chen CC, Johnson PA.
    Journal: Gen Comp Endocrinol; 1997 Sep; 107(3):386-93. PubMed ID: 9268619.
    Abstract:
    In order to understand the role of inhibin and activin in regulating follicular development in the hen, the steady-state mRNA levels of inhibin/activin alpha- and betaA-subunits in the granulosa layer of the largest (F1) and second largest (F2) follicles of the hen were investigated at 4-hr intervals throughout the ovulatory cycle. In addition, because it was hypothesized that luteinizing hormone (LH) regulated betaA-subunit expression, the effect of in vivo administration of ovine LH (oLH) on the expression of these subunits during the early- and mid-ovulatory cycle was examined. Northern blot analysis, using 32P-labeled cDNA probes of chicken inhibin/activin alpha- and betaA-subunits and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, internal control), revealed that in the F1 follicle, the relative level of betaA-mRNA (n = 3) was low at 23.5 hr and increased (P < 0.05) at 19.5, 15.5, and 11.5 hr before the next predicted ovulation. It then decreased (P < 0.05) at 7.5 hr and was further reduced at 3.5 and 0.5 hr prior to ovulation. In the F2 follicle, betaA-mRNA was maintained at a basal level throughout the sampling period except for a brief increase (P < 0.05) at 0.5 hr before ovulation. In contrast to the betaA-subunit, inhibin alpha-mRNA was abundantly expressed with no significant variations throughout the ovulatory cycle in either the F1 or the F2 follicle. When oLH was injected at 18 hr before ovulation, 200 but not 100 or 50 microg/kg (n = 3 hens per dose) significantly (P < 0.05) reduced the betaA-mRNA level in the F1 follicle by 2 hr after injection compared to the control (saline). The experiment was repeated at 12 hr before ovulation and both 100 and 200 but not 50 microg/kg oLH significantly (P < 0.05) reduced the expression of betaA-subunit mRNA with no significant difference between 100 and 200 microg/kg oLH. In contrast to the betaA-subunit, inhibin alpha-subunit mRNA was abundantly expressed and not affected by oLH treatment. Our data indicate that the expression of inhibin/activin betaA- but not alpha-subunit mRNA is developmentally regulated in the granulosa layer of the two largest follicles during the hen ovulatory cycle. In addition, LH may participate, directly or indirectly, in negative regulation of the betaA-subunit.
    [Abstract] [Full Text] [Related] [New Search]