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  • Title: The sequence of palF, an environmental pH response gene in Aspergillus nidulans.
    Author: Maccheroni W, May GS, Martinez-Rossi NM, Rossi A.
    Journal: Gene; 1997 Jul 31; 194(2):163-7. PubMed ID: 9272857.
    Abstract:
    To molecularly characterize the influence of external pH in the secretion of enzymes by filamentous fungi, we have cloned and sequenced the palF gene of Aspergillus nidulans (An). An An wild-type (wt) chromosome VII-specific cosmid library was used to transform a palF15 mutant strain. Selection for complementation was done on medium containing beta-glycerolphosphate as the sole Pi source. Two cosmids were identified (W2G08 and W4G12) and further subcloning of one cosmid (W2G08) defined a 5-kb PstI genomic fragment, which fully complements the palF15 mutation. An internal fragment from the genomic clone recognized a single message of approx. 3.5 kb on Northern blot. cDNA clones were obtained from a lambda gt10 cDNA library and sequenced, showing a nucleotide (nt) sequence of 3311 base pairs (bp), with a 828 bp long 5'-untranslated region (UTR). The major open reading frame (ORF) identified in the sequence codes for a putative 775 amino acid (aa) protein, which shares some similarity with two putative ORF products of Saccharomyces cerevisiae (Sc).
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