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  • Title: A new and improved "quick-hot Gram-chromotrope" technique that differentially stains microsporidian spores in clinical samples, including paraffin-embedded tissue sections.
    Author: Moura H, Schwartz DA, Bornay-Llinares F, Sodré FC, Wallace S, Visvesvara GS.
    Journal: Arch Pathol Lab Med; 1997 Aug; 121(8):888-93. PubMed ID: 9278620.
    Abstract:
    OBJECTIVE: This report describes a new and improved "quick-hot Gram-chromotrope" staining technique that detects microsporidian spores in clinical specimens, such as stool, urine, saliva, nasopharyngeal fluid, and bronchoalveolar lavage samples, as well as in formalin-fixed and paraffin-embedded tissue sections. DESIGN: In this procedure, the samples are stained in heated (50 degrees C to 55 degrees C) solutions of crystal violet and iodine used in Gram's stain, followed by a modified chromotrope solution (heated to 50 degrees C to 55 degrees C). The modified stain is composed of chromotrope 2R (1%), fast green (0.15%), and phosphotungstic acid (0.25%). RESULTS: With this stain and the new protocol, microsporidian spores are stained dark violet against a pale green background, and the total staining time is shortened to 5 minutes. CONCLUSIONS: This new technique is fast, reliable, and simple. It can be easily adapted for use in clinical laboratories.
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