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  • Title: [Clinical evaluation of RT-PCR method for detection of HCV-RNA--with special reference to Amplicor HCV].
    Author: Furuwatari C, Suzuki K, Matsumoto A, Kobayashi M, Kiyosawa K, Hidaka E, Ueno I, Ogiso Y, Furihata K, Katsuyama T.
    Journal: Rinsho Byori; 1997 Aug; 45(8):790-4. PubMed ID: 9283232.
    Abstract:
    Detection of the viral genome in serum is the most reliable way to analyze HCV viremia. In the present study, we evaluated the availability of a new detection kit for HCV-RNA, Amplicor HCV, which is based on the RT-PCR microplate hybridization protocol. The procedure of Amplicor HCV is simple, unlike the conventional RT-nested-PCR method. Although Amplicor HCV assay exhibited the lower sensitivity than the the conventional RT-nested-PCR method (10 x for HCV type 1a, 1b and 2b; 10(3) x for type 2a), Amplicor HCV assay could detect the HCV-RNA in all HCV-RNA positive cases by conventional RT-nested PCR method, except for one case who contained low concentration of HCV-RNA (10 copies/ml). The coincidence rate was 99.2% in 120 clinical samples between two assays. Amplicor HCV assay, moreover, could efficiently evaluate the viremia regardless of anti-HCV-2 antibody titer. This assay was useful for monitoring the effect of interferon therapy during and after administration. These results suggest that Amplicor HCV has an excellent availability for the clinical laboratories.
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