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  • Title: Isolation and characterization of myeloperoxidase from leukocytes of rat peritoneal fluid.
    Author: Morozov VI, Tsyplenkov PV, Kokryakov VN, Volkov KN, Vinogradova NA.
    Journal: Biochemistry (Mosc); 1997 Jun; 62(6):623-30. PubMed ID: 9284544.
    Abstract:
    Myeloperoxidase (MPO) was isolated from rat peritoneal leukocytes with a yield of 51% and A430/A280 = 0.75 - 0.80, and its physicochemical properties were studied. The molecular weight of the MPO is about 150 kD. The MPO was assayed for amino acid content. We used substrate mixture containing phenol, 4-aminoantipyrine, and H2O2 to detect 10(-10) M of the enzyme. The MPO was localized in rat blood neutrophils using polyclonal anti-MPO antibodies and secondary fluorescein isothiocyanate-labeled antibodies. Immunofluorimetric assay (IFMA) was developed for quantitative measurement of the MPO. The MPO and leukocytes can iodinate BSA using NaI or thyroxine as the source of iodine.
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