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  • Title: Arachidonic acid release from rat Leydig cells depends on the presence of luteinizing hormone/human chorionic gonadotrophin receptors.
    Author: Moraga PF, Llanos MN, Ronco AM.
    Journal: J Endocrinol; 1997 Aug; 154(2):201-9. PubMed ID: 9291830.
    Abstract:
    In this work, the involvement of arachidonic acid (AA) in the luteinizing hormone and human chorionic gonadotrophin (LH/hCG) action on Leydig cells was studied. Experiments were first designed to evaluate [14C]AA incorporation into membrane phospholipids. Subsequently, time-course, pulse-chase and dose-response studies of the effect of hCG on [14C]AA release were performed. Results indicated that 4 h was optimal for maximal incorporation of [14C]AA into membrane phospholipids of viable Leydig cells. Pulse-chase experiments and studies performed to evaluate the effect of different doses of hCG on [14C]AA release demonstrated that this hormone stimulates [14C]AA release in a dose-response and time-dependent manner. Furthermore, using a desensitised animal model, a link between the presence of LH/hCG receptors and LH/hCG-stimulated [14C]AA release in Leydig cells could be established. In fact, the amount of [14C]AA released was significantly dependent on, and directly proportional to, the concentration of LH/hCG binding sites. Thus [14C]AA released from intact rat Leydig cells decreased when animals had been previously injected with a high single dose of hCG (desensitised animals), which is known to cause a dramatic decrease in the number of LH/hCG binding sites. These results demonstrate that the mechanism of AA release in Leydig cells depends on LH/hCG-receptor interaction and also suggest that AA could act as an additional intracellular messenger associated with the hormonal action of LH/hCG.
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