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  • Title: A binding assay for 25-hydroxycalciferols and 24R,25-dihydroxycalciferols using bovine plasma globulin.
    Author: Hollis BW, Burton JH, Draper HH.
    Journal: Steroids; 1977 Aug; 30(2):285-93. PubMed ID: 929649.
    Abstract:
    The concentrations of the 25-hydroxy and 24R, 25-dihydroxy derivatives of vitamin D were determined in 100 microliter l plasma samples using calciferol binding globulin from bovine plasma. Sufficient quantities of 24R, 25-dihydroxy vitamin D were found in bovine, porcine, chicken and human plasma to interfere in the assay of 25-hydroxy vitamin D in unfractionated extracts. No metabolites of vitamin D could be found in rainbow trout plasma.
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