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  • Title: Detection of triazolam in skeletal remains buried for 4 years.
    Author: Kudo K, Sugie H, Syoui N, Kurihara K, Jitsufuchi N, Imamura T, Ikeda N.
    Journal: Int J Legal Med; 1997; 110(5):281-3. PubMed ID: 9297586.
    Abstract:
    Analyses of the hypnotic triazolam from the remains of two human skeletons buried underground for 4 years were made for purposes of confirmation. The bone marrow and mummified muscle were digested with 2 M sodium hydroxide, efficiently extracted using a 3-step solvent extraction procedure, and selectively analyzed by gas chromatography/mass spectrometry with the negative ion chemical ionization mode. Estazolam was the internal standard used. Triazolam was detected in all the samples; the concentrations were 0.36 ng/g in the bone marrow of one victim, and 0.37 and 5.5 ng/g in the bone marrow and mummified muscle of the other victim. This method should prove useful for determination of triazolam in extensively decomposed bodies.
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