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  • Title: Rescue of a viral gene from VERO cells latently infected with influenza virus B/Lee/40.
    Author: Tobita K, Tanaka T, Hayase Y.
    Journal: Virology; 1997 Sep 15; 236(1):130-6. PubMed ID: 9299625.
    Abstract:
    By growing VERO cells infected with 5 PFU/cell of influenza virus B/Lee/40, a latently infected culture was readily established (L/V cells). The cells continued to multiply stably, excreting a small amount of virus in the beginning, which sharply declined according to cell division to undetectable level by day 9. However, nucleotide sequences for all the 8 genes of B/Lee/40 as well as their mRNAs were amplified from L/V cells on day 50 or later by RT-PCR. Moreover, from the 95-day-old L/V cells, a persisting NP gene of B/Lee/40 was rescued into infectious virus particles upon superinfection with homotypic influenza virus B/Yamagata/1/73.
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