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  • Title: [Electrophoretic characteristics of the proteins and the preparative production of neuraminidase from the A/turkey/Wisconsin/66 (Hav6N2) strain of the avian influenza virus].
    Author: Zakstel'skaia LIa, Ivanova VT, Molibog EV.
    Journal: Vopr Virusol; 1977; (5):551-7. PubMed ID: 930051.
    Abstract:
    A combination of electrophoresis in a single plate of polyacrylamide gel with preparative production of subunits upon electrophoresis in acetate cellulose was used for the analysis of the polypeptide composition of the A/turkey/Wisconsin/66 strain. Seven classes of proteins with certain functional significance and 4 minor components were detected. A preparation of neuraminidase could be obtained which has enzymatic and antigenic activity, and a molecular mass of 68,000 daltons. Some features of the polypeptide composition were revealed, including the presence of uncleaved hemagglutinin under reducing conditions and a decreased content of the light chain of hemagglutinin.
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