These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Use of the ASTED system to determine L-NG-monomethylarginine (546C88) in human plasma by pre-column o-phthalaldehyde derivatisation and high-performance liquid chromatography.
    Author: Cooper JD, Shearsby N, Sheung CT.
    Journal: J Chromatogr B Biomed Sci Appl; 1997 Aug 15; 696(1):117-22. PubMed ID: 9300915.
    Abstract:
    The use of the system automated sequential trace enrichment of dialysates (ASTED), to prepare plasma samples for the estimation of L-NG-monomethylarginine (546C88) by pre-column o-phthalaldehyde-2-mercaptoethanol derivatisation and HPLC is described. Calibration is achieved using purified albumin as a substitute matrix for plasma. Using this technique the procedure was observed to be specific for 546C88 and linear over the range 0.10 to 50.0 mumol/l. The within-run imprecision (C.V.) at four different spiked plasma 546C88 concentrations of 0.10, 1.0, 8.0 and 40.0 mumol/l was 6.48, 2.55, 2.79 and 3.37%, respectively, and the between-run imprecision (C.V.) estimated to be 8.50, 1.80, 2.10 and 3.30%, respectively, for the same spiked 546C88 concentrations. The overall accuracy (% bias) of the procedure using an albumin matrix for calibration was estimated to be -2.50, -5.25, -3.56, -3.53%, respectively, and the recovery of 546C88 from six different spiked plasma samples estimated to be 99.1 +/- 1.4%.
    [Abstract] [Full Text] [Related] [New Search]