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  • Title: Tetraphenylphosphonium inhibits oxidation of physiological substrates in heart mitochondria.
    Author: Mildaziene V, Baniene R, Marcinkeviciute A, Nauciene Z, Kalvenas A, Zimkus A.
    Journal: Mol Cell Biochem; 1997 Sep; 174(1-2):67-70. PubMed ID: 9309667.
    Abstract:
    We show that tetraphenylphosphonium inhibits oxidation of palmitoylcarnitine, pyruvate, malate, 2-oxoglutarate and glutamate in heart mitochondria in the range of concentration (1-5 microM) commonly used for the determination of mitochondrial membrane potential. The inhibition of 2-oxoglutarate (but not other substrate) oxidation by tetraphenylphosphonium is dependent on the concentration of 2-oxoglutarate and on extramitochondrial free calcium, and the kinetic plots are consistent with a mixed type of inhibition. Our results indicate that tetraphenylphosphonium interacts with enzymes, specifically involved in the oxidation of 2-oxoglutarate, most possibly, 2-oxoglutarate dehydrogenase.
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