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  • Title: Specificity of cytochemical procedures for localising peroxidase activity in the sarcoplasmic reticulum.
    Author: Christie KN, Stoward PJ.
    Journal: Histochemistry; 1979; 64(3):315-8. PubMed ID: 93104.
    Abstract:
    Cytochemical evidence is reported for substantiating the view that when lightly-fixed skeletal muscle is incubated in a diaminobenzidine-H2O2 medium at pH 5, the resulting enhanced electron opacity of the sarcoplasmic reticulum is more likely to be due to a peroxidatic activity therein rather than to a non-enzymic binding reaction. The reticulum staining is absent in incubated sections of overfixed or boiled tissue; or if hydrogen peroxide is omitted from the incubation medium; or if aminotriazole is included in the medium.
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