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  • Title: Simple, direct determination of serum 5'-nucleotidase.
    Author: Baginski ES, Marie SS, Epstein E, Zak B.
    Journal: Ann Clin Lab Sci; 1977; 7(6):469-78. PubMed ID: 931350.
    Abstract:
    A simple determination of 5'-Nucleotidase in blood serum without deproteinization is described. The enzyme activity is distinguished from that of a nonspecific alkaline phosphatase by nickel inhibition and the inorganic phosphate released from adenosine monophosphate used as substrate is determined by a method previously described. Additional studies include the determination of optimal conditions for the reaction and nickel inhibition.
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