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  • Title: Oxygen requirement for acetylene reduction by pure cultures of rhizobia.
    Author: Keister DL, Evans WR.
    Journal: J Bacteriol; 1976 Jul; 127(1):149-53. PubMed ID: 931945.
    Abstract:
    The oxygen and nutritional requirements for acetylene reduction by Rhizobium japonicum and Rhizobium sp. in liquid culture are described. The optimal oxygen concentration was about 0.1% in the gas phase, which is lower than that of any other known aerobic nitrogen-fixing microorganism. these organisms are also unique in that nitrogenase synthesis is not repressed in the presence of ammonium chloride under certain cultural conditions, in contrast to other wild-type bacteria.
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