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  • Title: Derivatization of compounds at the origin of thin-layer plates with fluorescamine.
    Author: Nakamura H, Pisano JJ.
    Journal: J Chromatogr; 1976 Jun 09; 121(1):33-40. PubMed ID: 932133.
    Abstract:
    A method has been developed for the detection of compounds with primary amino groups on thin-layer chromatography (TLC) plates with fluorescamine. The compounds dissolved in buffer were applied to TLC plates and derivatized at the origin by developing with or dipping into an acetone-hexane solution of fluorescamine. Virtually all fluorescamine derivatives stayed at the origin, and they were subsequently separated using appropriate solvent systems. As little as 10 pmoles of fluorescamine-derivatized compound could be detected when viewed under a long-wave (366 nm) ultraviolet lamp. The method was applied to the analysis of peptides, amino acids and amines.
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