These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Lack of correlation between growth of TF-1 cells and tyrosine phosphorylation signals in response to IL-3, IL-5 and GM-CSF.
    Author: Ettinger S, Fong D, Duronio V.
    Journal: Cytokine; 1997 Sep; 9(9):650-9. PubMed ID: 9325013.
    Abstract:
    The human cell line, TF-1, was used to compare responses to interleukin 3 (IL-3), IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF). TF-1 cells grew well in the presence of any one of the cytokines in early passages. However, the level of tyrosine phosphorylation was minimal in response to IL-5, and detection of a tyrosine phosphorylation signal required high concentrations of IL-5. When grown for longer periods of time in the presence of one of the cytokines, there were dramatic difference in the cells' responses. IL-3 or GM-CSF-grown cells showed only half of the original bioassay response to IL-5. However, cells grown in IL-5 alone kept the same response, and all cells showed the same response to IL-3 and GM-CSF. IL-5-grown cells also had an increased tyrosine phosphorylation signal, along with increased sensitivity to IL-5, yet there was no difference in an IL-5 bioassay. The relative level of detection of tyrosine phosphorylated JAK-2, STAT-5, SHC, and other substrates corresponded to the overall tyrosine phosphorylation signal. IL-5-grown cells had approximately 10-fold more IL-5 receptor alpha subunit message compared to IL-3-grown. These results suggest that response of TF-1 cells to IL-5 may be deceiving in that a good response in a bioassay can be observed with relatively little tyrosine phosphorylation, but an increase in tyrosine phosphorylation can be correlated with an increase in the expression of IL-5 receptor alpha subunit.
    [Abstract] [Full Text] [Related] [New Search]