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  • Title: Isolation of osteosarcoma-associated amplified DNA sequences using representational difference analysis.
    Author: Simons A, Janssen IM, Suijkerbuijk RF, Veth RP, Pruszczynski M, Hulsbergen-van de Kaa CA, du Manoir S, Geurts van Kessel A.
    Journal: Genes Chromosomes Cancer; 1997 Oct; 20(2):196-200. PubMed ID: 9331570.
    Abstract:
    Comparative genomic hybridization analysis of a primary osteosarcoma and its metastasis revealed two regions of DNA amplification, one at 17p11.2-12 and one at 19q12-13. Subsequent representational difference analysis of the primary tumor resulted in the isolation of two distinct tumor-amplified DNA fragments originating from chromosome 19. A YAC clone corresponding to one of the two isolated DNA fragments was used for fluorescence in situ hybridization on normal human lymphocyte metaphases and tumor-derived nuclei. This resulted in the localization of this YAC to 19q12-13.1 and confirmed the amplification status of the isolated fragment in the tumors. The availability of such RDA-isolated sequences may be instrumental in the search for genes relevant for tumor development.
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