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  • Title: Homology-dependent silencing of the SC3 gene in Schizophyllum commune.
    Author: Schuurs TA, Schaeffer EA, Wessels JG.
    Journal: Genetics; 1997 Oct; 147(2):589-96. PubMed ID: 9335596.
    Abstract:
    After introduction of extra copies of the SC3 hydrophobin gene into a wild-type strain of Schizophyllum commune, gene silencing was observed acting on both endogenous and introduced SC3 genes in primary vegetative transformants. Nuclear run-on experiments indicated that silencing acted at the transcriptional level. Southern analysis revealed that cytosine methylation of genomic DNA occurred. Moreover, SC3 silencing was suppressed by exposure to 5-azacytidine during growth. After growth of SC3-suppressed colonies from homogenized mycelium or from colonies stored at 4 degrees, SC3 transcription was restored. However, after prolonged growth SC3 silencing was again observed. Introduction of a promoterless SC3 fragment into wild type gave less SC3 silencing.
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