These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Evidence for the essential histidine residues in geranylgeranyl transferase type I from bovine testis.
    Author: Kim H, Yoon BY, Yang CH.
    Journal: Biochem Mol Biol Int; 1997 Oct; 43(2):453-62. PubMed ID: 9350353.
    Abstract:
    Geranylgeranyl transferase was purified 30,000-fold by sequential use of 30-50% ammonium sulfate fractionation, Q-Sepharose anion exchange chromatography, Phenyl Superose hydrophobic interaction chromatography, Sephacryl S-300 gel filtration chromatography, and peptide (YREKKFFCAIL) affinity chromatography. Geranylgeranyl transferase, when incubated with diethyl pyrocarbonate (DEPC), a histidine-specific reagent, shows time-dependent inactivation, and the activity is restored by the addition of neutral hydroxylamine. The inactivation follows pseudo-first order kinetics with a second order rate constant of 0.319 M-1min-1. The overall results thus provide evidence that a histidine residue in the active site is involved in the catalytic mechanism of the geranylgeranyl transferase reaction.
    [Abstract] [Full Text] [Related] [New Search]