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  • Title: Immunohistochemical analysis of heme oxygenase-I in rat liver after ischemia.
    Author: Ito K, Ikeda S, Shibata T, Yano T, Horikawa S.
    Journal: Biochem Mol Biol Int; 1997 Oct; 43(3):551-6. PubMed ID: 9352073.
    Abstract:
    Total hepatic ischemia was induced by clamping the hepatic artery, portal vein, and bile duct. After 15 min hepatic ischemia, we examined a time course of the changes in the steady-state levels of heme oxygenase-1 (HO-1) mRNA and protein in the livers. The levels of HO-1 mRNA was transiently induced and peaked at 4 h after the start of reperfusion. In addition, a pattern of the time course of HO-1 protein levels was similar to that of HO-1 mRNA levels. Immunohistochemical analysis clearly showed that the localization of HO-1 protein induced by hepatic ischemia was restricted to the hepatocytes in the pericentral vein.
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