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  • Title: [Determination of lecithin: cholesterol acyltransferase activity by a radioisotopic assay (author's transl)].
    Author: Ras MR, Masdeu S, Miquel C, Frisón JC, Rubiés-Prat J, Schwartz S.
    Journal: Rev Esp Fisiol; 1976 Jun; 32(2):95-8. PubMed ID: 935626.
    Abstract:
    A method for determining Lecithin: Cholesterol Acyltransferase (LCAT) activity is presented. Trace amounts of labelled cholesterol are added to innactivated homologous substrate from a pool of normal serum. LCAT activity is determined measuring the cholesterol esterification, and is expressed as micrograms of cholesterol esters formed per milliliter plasma per hour. LCAT time-course, reproducibility, activity after serum storage at 4 degrees C, and normal values from 20 healthy subjects are studied.
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