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  • Title: Comparison of two methods in erythrocyte microrheology determination using glutaraldehyde-treated cells.
    Author: Mirossay L, Mojzis J, Jandoseková M, Lukacín S, Nicák A.
    Journal: Clin Hemorheol Microcirc; 1997; 17(3):187-92. PubMed ID: 9356782.
    Abstract:
    The change in human red blood cell microrheology in different glutaraldehyde concentrations (1.5, 3.0 and 5.0 x 10(-6) mol l-1) was studied. The method of millipore filtration was compared with the method of cation-osmotic hemolysis. The obtained results revealed that the prolongation of the erythrocyte filtration time correlated with the curve shifts found in cation-osmotic hemolysis. Contrary to the filtration method, significant differences between two lower concentrations of glutaraldehyde (1.5 and 3.0 x 10(-6) mol l-1) were found. Therefore, we conclude that the cation-osmotic hemolysis is more sensitive than the filtration method in determining the red blood cell deformability.
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