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  • Title: Regionalized expression of CD52 in rat epididymis is related to mRNA poly(A) tail length.
    Author: Pera I, Derr P, Yeung CH, Cooper TG, Kirchhoff C.
    Journal: Mol Reprod Dev; 1997 Dec; 48(4):433-41. PubMed ID: 9364437.
    Abstract:
    The regional pattern of CD52 expression in the rat epididymis was followed by Northern analyses and carbohydrate-labelling of glycoconjugates on Western blots. CD52 mRNA showed a novel aspect of regionalization, namely region-dependent length differences in its poly(A) tail. 'Short' CD52 mRNA molecules were present in all parts of this organ and also in the seminal vesicles. Additionally, the cauda epididymidis contained mRNA molecules with an extended poly(A) tail. Their appearance coincided with the occurrence of the principal M(r) approximately 26 kDa glycopeptide in the cauda region, representing the CD52 product. CD52 expression seemed to be regulated or modulated synergistically by androgens, temperature, and (an) unknown testicular factor(s), depending on the poly(A) tail length of its mRNA. Androgens alone exerted an effect only on molecules with 'short' poly(A) tails. They were down-regulated in castrated animals, and restored to normal levels upon testosterone supplementation. However, 'long' CD52 mRNA molecules were not affected. Only if combined with the exposure of the epididymis to the elevated temperature of the abdomen, castration of animals resulted in a complete loss of the CD52 mRNA, including the 'long' cauda species. Loss of 'long' CD52 mRNA molecules was also observed when the abdominal location was combined with efferent duct ligation. This combination of treatments, however, did not affect 'short' CD52 mRNA levels. Loss of the 'long' CD52 mRNA molecules by any treatment coincided with a loss of the principal M(r) approximately 26 kDa glycopeptide from caudal protein extracts.
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