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  • Title: High-performance liquid chromatographic determination of oxolinic acid in the plasma of seabass (Dicentrarchus labrax) anaesthetized with 2-phenoxyethanol.
    Author: Loussouarn S, Pouliquen H, Armand F.
    Journal: J Chromatogr B Biomed Sci Appl; 1997 Sep 26; 698(1-2):251-9. PubMed ID: 9367215.
    Abstract:
    A high-performance liquid chromatographic (HPLC) method for the determination of oxolinic acid (OA) in the plasma of seabass (Dicentrarchus labrax) anaesthetized with 2-phenoxyethanol is described. The samples were extracted and cleaned up by a solid-phase extraction procedure using C18 extraction cartridges. After the eluent was evaporated, the dry residue was dissolved in 1/15 M phosphate buffer. OA was determined by using an isocratic HPLC method with UV detection at 340 nm. Seabass drug-free plasma samples were spiked with OA at 0.2, 1.0, 5.0 and 25.0 microg/ml. Validation of the method showed good precision and accuracy. The mean recovery was 92.2%, with a relative standard deviation lower than 5%. The quantification limit was 0.2 microg/ml. The method was tested on 300 plasma samples of OA-treated seabass.
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