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  • Title: Low dose-rate X-irradiation induces larger deletions at the human HPRT locus than high dose-rate X-irradiation.
    Author: Colussi N, Lohman PH.
    Journal: Int J Radiat Biol; 1997 Nov; 72(5):531-6. PubMed ID: 9374433.
    Abstract:
    PURPOSE: To investigate the size of deletions induced by low dose-rate (LDR) X-irradiation in relation to the induction by high dose-rate (HDR) X-irradiation at the HPRT locus of human lymphoblastoid WI-L2-NS cells. MATERIALS AND METHODS: The molecular nature of HPRT deletions in human WI-L2-NS lymphoblasts was investigated after LDR or HDR X-irradiation with a total dose of 4 Gy using a set of nine Xq26 sequence tagged sites (STS loci) markers surrounding the hprt gene and covering approximately 2.75 Mb. RESULTS: Eleven of 24 LDR X-ray-induced mutants contained a deletion of the entire hprt gene, whereas this was the case in 10 of 19 HDR X-irradiation-induced mutants. Most of the LDR and HDR total gene deletions exhibited also loss of markers adjacent to the HPRT locus, 10/11 and 7/10 respectively. The largest HDR X-irradiation induced deletion was 0.89 Mb, while the largest deletion observed after LDR X-irradiation (4/11 HPRT deletion mutants) extended outside the investigated region in both proximal (> 0.57 Mb) and distal directions (> 2 Mb). CONCLUSIONS: The average size of HPRT deletions after LDR X-irradiation was significantly larger (p = 0.0024) than following HDR, suggesting that an inverse dose-rate effect exists for the size of deletions induced by X-irradiation.
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