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  • Title: Stabilization of human serum alkaline phosphatase to histidine-induced heat inactivation by tryptic digestion.
    Author: Yamashita T, Sawanobori-isobe E, Mori M.
    Journal: J Biochem; 1976 Jul; 80(1):129-34. PubMed ID: 9376.
    Abstract:
    1. Serum alkaline phosphatase [EC 3.1.3.1] was strongly inactivated by histidine during incubation at pH 8.0 and 45degrees; however, tryptic digestion of the serum strongly protected the enzyme against inactivation by histidine. In the absence of histidine, however, neither heat inactivation of the phosphatase nor the effect of trypsin [EC 3.4.21.4] was observed. Factors affecting the alkaline phosphatase inactivation were studied further. 2. The effect of trypsin on the histidine-induced heat inactivation differed considerably according to the tissue source of the enzyme, which suggests a possible method for distinguishing alkaline phosphatase isoenzymes.
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