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  • Title: Identification of Chlorella T-1 ingredient which enhances DMPO-OH adduct formation in Fenton reaction.
    Author: Ali MA, Oykawa K, Ohwada M, Konishi T.
    Journal: Biochem Mol Biol Int; 1997 Nov; 43(4):787-97. PubMed ID: 9385439.
    Abstract:
    Chlorella T-1 is a micro algae which carries out highly active photosynthetic carbon fixation. For searching the OH scavenging molecules in this strain, the methanol extract of T-1 was fractionated by RP-HPLC and the fractions were analyzed for DMPO-OH formation by ESR after Fenton reaction. In the course of study, a single chlorella T-1 ingredient was found to enhance the formation of DMPO-OH rather than scavenge in the Fenton system. The OH enhancing chlorella component was further purified by chromatography and finally identified as lactic acid by NMR and LC-MS. From the analysis of the reaction conditions for DMPO-OH, it was found that lactic acid did not serve as direct source of OH but accelerated Fenton reaction.
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