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  • Title: Energetics and chronology of phototransients in the light response of the purple membrane of Halobacterium halobium.
    Author: Sherman WV, Korenstein R, Caplan SR.
    Journal: Biochim Biophys Acta; 1976 Jun 08; 430(3):454-8. PubMed ID: 938642.
    Abstract:
    Arrhenius parameters for formation and decay of phototransients in suspensions of purple membrane fragments in H2O and 2H2O have been determined in the temperature range 0-60 degree C. Kinetic isotope effects are found which show that proton transfer steps are involved in both formation and decay of the two longest-lived transients absorbing at 410 nm and 660 nm, respectively. The results also suggest that these transients do not occupy a single pathway in the spontaneous deexcitation of bacteriorhodopsin within the purple membrane. Purple membrane undergoes a phase transition at 25-30 degrees C in both H2O and 2H2O.
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